制革污泥渗滤液中特征污染物对土壤氨氮转化及微生物群落结构的影响

孔祥科; 李义; 王平; 韩占涛; 刘圣华; 张兆吉; 王妍妍

doi:10.12029/gc20220312001

制革污泥渗滤液中特征污染物对土壤氨氮转化及微生物群落结构的影响

1.
中国地质科学院水文地质环境地质研究所, 福建厦门 361021

2.
河北省/中国地质调查局地下水污染机理与修复重点实验室, 河北石家庄 050061

3.
河北省地质环境监测院, 河北石家庄 050021

4.
生态环境部土壤与农业农村生态环境监管技术中心, 北京 100012

基金项目: 河北省自然科学基金面上项目（D2020504003）及中国地质科学院基本科研业务费项目（SK202113）联合资助。

详细信息

作者简介: 孔祥科，男，1987年生，副研究员，从事土壤、地下水污染机理与修复研究；E-mail：kongxiangke@mail.cgs.gov.cn

通讯作者: 王妍妍，女，1987年生，副研究员，主要从事地下水污染机理研究；E-mail：wangyanyan@mail.cgs.gov.cn。

中图分类号: X794; X53

收稿日期: 2022-03-12

修回日期: 2022-04-26

刊出日期: 2024-09-25

Effects of characteristic pollutants in leather sludge leachate on soil ammonia nitrogen transformation and microbial community structures

1.
Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Xiamen 361021, Fujian, China

2.
Key Laboratory of Groundwater Remediation of Hebei Province and China Geological Survey, Shijiazhuang 050061, Hebei, China

3.
Hebei Geological Environment Monitoring Institute, Shijiazhuang 050021, Hebei, China

4.
Technical Centre for Soil, Agriculture and Rural Ecology and Environment, Ministry of Ecology and Environment, Beijing 100012, China

Fund Project: Supported by Natural Science Foundation of Hebei Province (No.D2020504003) and the Basic Scientific Research Fund of Chinese Academy of Geological Sciences (No.SK202113).

More Information

Author Bio: KONG Xiangke, male, born in 1987, associate researcher, engaged in the mechanism and remediation of soil and groundwater pollution; E-mail:kongxiangke@mail.cgs.gov.cn .

Corresponding author: WANG Yanyan, female, born in 1987, associate researcher, engaged in the mechanism of groundwater pollution; E-mail: wangyanyan@mail.cgs.gov.cn.

Received Date: 12 March 2022

Revised Date: 26 April 2022

Publish Date: 25 September 2024

摘要

摘要: 研究目的
查明制革污泥渗滤液中特征污染物（Cr(III)、盐分、有机质）对土壤NH₄⁺−N转化和微生物群落结构的影响，为制革污泥的安全农用及土壤NH₄⁺−N的污染防控提供理论依据。
研究方法
通过土壤培养实验，研究不同污染物影响下土壤中NH₄⁺−N向NO₃⁻−N的转化规律，并利用高通量测序对比分析不同条件下的微生物群落组成和结构变化特征。
研究结果
制革污泥渗滤液中特征污染组分对土壤中NH₄⁺−N硝化过程的抑制程度排序为：Cr(III)>盐分>有机质。外源Cr(III)进入对土壤中NH₄⁺−N的转化速率和转化量具有明显抑制作用，而土壤中盐分和有机质主要影响硝化作用的启动时间和降低硝化速率。随着土壤中Cr(III)含量由100 mg/kg增加到250 mg/kg，培养90 d后NH₄⁺−N的转化量由94.23%降低到19.38%。高浓度Cr(III)和盐分在污染初期对土壤中微生物丰度和群落结构影响显著，随土壤老化过程中Cr(III)生物有效性降低及微生物适应性增强，不同污染特征土壤中微生物群落结构和组成逐渐趋同，硝化功能菌（Nitrosospira、Nitrosomonas和Nitrosospira）丰度增加明显。
结论
冗余（RDA）分析结果表明，影响制革污染土壤中微生物群落结构演变的主要特征因子是Cr(III)（R²=0.53，P<0.01）、NH₄⁺−N（R²=0.59，P<0.005）和NO₃⁻−N（R²=0.53，P<0.01）。
- 制革污泥 /
- Cr(III) /
- 氨氮 /
- 硝化作用 /
- 微生物群落结构 /
- 土壤环境地质调查工程
Abstract:
This paper is the result of soil environmental survey engineering.
Objective
The objective is to identify the impact of characteristic pollutants (Cr (III), salt, organic matter) in the leachate of tannery sludge on the conversion of soil NH₄⁺−N conversion and microbial community structure. It provides a theoretical basis for the safe agricultural use of tannery sludge and soil pollution prevention and control.
Methods
The transformation of NH₄⁺−N to NO₃⁻−N in soil was studied through soil cultivation experiments under different pollution conditions, and the high−throughput sequencing is used to analyze the composition and structure characteristics of microbial community in soil.
Results
The characteristic pollution components in the tannery sludge leachate inhibit the nitrification process of NH₄⁺−N in the soil, and the influence factors are sorted as follows: Cr(III)>saltness>organic matter. The exogenous Cr(III) pollution has a significant inhibitory effect on the transformation rate and amount of NH₄⁺−N, whereas the saltness and organic matter only delay the start time of nitrification and reduce the nitrification rate in soil. With the increase of Cr(III) content from 100 mg/kg to 250 mg/kg, the transformation amount of NH₄⁺−N decreases from 94.23% to 19.38% after 90 days of culture. The high Cr(III) and salinity in the leachate have a significant impact on the microbial community structure and distribution in the soil at the initial stage of pollution. With the decrease of the bioavailability of Cr(III) and the enhancement of microbial adaptation in soil aging process, the microbial community structure and composition in Cr(III), salinity, and organic matter contaminated soils become similar, and the abundances of nitrifying bacteria (Nitrosospira, Nitrosomonas and Nitrosospira) also have an obvious increase.
Conclusions
The results of RDA analysis show that the main characteristic factors affecting the evolution of microbial community structure in tanning contaminated soil are: Cr(III) (R²=0.53, P<0.01), NH₄⁺−N (R²=0.59, P<0.005) and NO₃⁻−N(R²=0.53, P<0.01).
- tannery sludge /
- Cr(III) /
- ammonia nitrogen /
- nitrification /
- microbial community structure /
- soil environmental survey engineering

HTML全文

图 1 不同环境条件下土壤中氨氮、亚硝酸盐及硝酸盐含量变化

Figure 1.

下载: 全尺寸图片幻灯片

图 2 不同环境条件下硝酸盐累积过程的Sigmodial拟合曲线

Figure 2.

下载: 全尺寸图片幻灯片

图 3 样本菌群分型图（a—韦恩图；b—聚类分布图；c—门水平组成图）

Figure 3.

下载: 全尺寸图片幻灯片

图 4 属分类水平上的微生物群落组成丰度图

Figure 4.

下载: 全尺寸图片幻灯片

图 5 微生物群落与环境因子的RDA分析

Figure 5.

下载: 全尺寸图片幻灯片

表 1 不同实验条件下土壤中污染组分含量

Table 1. Contents of contaminated components in soil under different experimental conditions

样品分组	NH₄⁺−N/ (mg/kg)	Cr(III)/ (mg/kg)	含盐量/ (mg/kg)	有机质/ %
CK	320.5	66.8	717	0.14
Cr1	316.3	103.5	1450	0.16
Cr2	318.0	252.8	2070	0.17
OM	319.7	69.8	885	0.42
Sal	318.4	67.2	10250	0.17
TS	268.5	184.0	10200	0.34

下载: 导出CSV

参考文献(50)

[1]	Cao X H, Guo J, Mao J D, Lan Y Q. 2011. Adsorption and mobility of Cr(III)–organic acid complexes in soils[J]. Journal of Hazardous Materials, 192(3): 1533−1538. doi: 10.1016/j.jhazmat.2011.06.076
[2]	Caporaso J G, Kuczynski J, Stombaugh J, Bittinger K, Bushman F D, Costello E K, Fierer N, Antonio G P, Goodrich J K, Gordon J I, Huttley G A, Kelley S T, Knights D, Koenig J E, Ley R E, Lozupone C A, McDonald D, Muegge B D, Pirrung M, Reeder J, Sevinsky J R, Turnbaugh P J, Walters W A, Widmann J, Yatsunenko T, Zaneveld J, Knight R. 2010. QIIME allows analysis of high–throughput community sequencing data[J]. Nature Methods, 7(5): 335−336. doi: 10.1038/nmeth.f.303
[3]	Chang Hao. 2014. Study on the Pyrolysis Process and Product Properties of Leather Sludge[D]. Xi'an: Shaanxi University of Science and Technology, 1−52 (in Chinese with English abstract).
[4]	Daims H, Lücker S, Wagner M. 2016. A new perspective on microbes formerly known as nitrite–oxidizing bacteria[J]. Trends in Microbiology, 24(9): 699−712. doi: 10.1016/j.tim.2016.05.004
[5]	Huang Xuefen, Meng Min, Xie Gang, Luo Yucheng, Li Lei, Wang Weisheng. 2017. Study on speciation distribution of Cr and reduction of Cr(Ⅵ) in tannery sludge[J]. Journal of Guangxi University (Natural Science Edition), 42(5): 1930−1936 (in Chinese with English abstract).
[6]	Ke X B, Angel R, Lu Y H, Conrad R. 2013. Niche differentiation of ammonia oxidizers and nitrite oxidizers in rice paddy soil[J]. Environmental Microbiology, 15: 2275−2292. doi: 10.1111/1462-2920.12098
[7]	Kong X K, Li C H, Wang P, Huang G X, Li Z T, Han Z T. 2019. Soil pollution characteristics and microbial responses in a vertical profile with long–term tannery sludge contamination in Hebei, China[J]. International Journal of Environmental Research and Public Health, 16(4): 563.
[8]	Kong X K, Wang Y Y, Ma L S, Li H, Han Z T. 2022. Impact of δ–MnO₂ on the chemical speciation and fractionation of Cr(III) in contaminated soils [J]. Environmental Science and Pollution Research, 29: 45328−45337.
[9]	Lai Qiaohui, Zhang Hao, Liu Zhipeng. 2019. Characteristics of soil microbial community and their impact factors in different constructed wetlands with plant communities[J]. Research of Soil and Water Conservation, 26(5): 89−94,99 (in Chinese with English abstract).
[10]	Li Juan, Uwaremwe Constantine, Leng Yan, Zhang Xiaohua, Li Shiweng, Chen Ximing. 2017. Progess on the study of biodegradation of organic pollutants and adsorption of heavy metals with arthrobacter strains[J]. Environmental Science & Technology, 40(10): 89−97 (in Chinese with English abstract).
[11]	Li Meng. 2017. Effect of Combined Application of Manure with Nitrogen Fertilizer on the Diversity of Nitrifier in Installed Vegetable Fields[D]. Shenyang: Shenyang Agricultural University, 1–56 (in Chinese with English abstract).
[12]	Liu Y C, Zhang Z J, Li Y S, Fei Y H. 2017. Response of soil microbial communities to roxarsone pollution along a concentration gradient[J]. Journal of Environmental Science & Health Part A Toxic/Hazardous Substances & Environmental Engineering, 52(9): 819−827.
[13]	Lü Xiaoli, Liu Jingtao, Han Zhantao, Zhu Liang, Yang Mingnan, Li Haijun. 2021. Geochemical characteristics and driving factors of high–ammonium groundwater in the rapid urbanization of the Pearl River Delta[J]. Geology in China, 48(6): 1770−1780 (in Chinese with English abstract).
[14]	Ma Hongrui, Wang Xiaorong, Niu Xiaojun, Li Guiju. 2004. Releases of Cr(Ⅲ) and nitrogen from contaminated soil with tannery sludge during organic matter mineralization[J]. Journal of Agro–Environment Science, 23(1): 136−139 (in Chinese with English abstract).
[15]	Martines A M, Nogueira M A, Santos C A, Nakatani A S, Andrade C A, Coscione A R, Cantarella H, Sousa J P, Cardoso E J B N. 2010. Ammonia volatilization in soil treated with tannery sludge[J]. Bioresource Technology, 101(12): 4690−4696. doi: 10.1016/j.biortech.2010.01.104
[16]	Nakatani A S, Martines A M, Nogueira M A, Fagotti D S L, Oliveira A G, Bini D, Sousa J P, Cardoso E J B N. 2011. Changes in the genetic structure of bacteria and microbial activity in an agricultural soil amended with tannery sludge[J]. Soil Biology and Biochemistry, 43(1): 106−114. doi: 10.1016/j.soilbio.2010.09.019
[17]	Pang Yajie, Li Chunhui, Han Zhantao, Zhang Zhaoji, Kong Xiangke. 2024. Migration simulation and pollution assessment of Cr (III) and ammonia from tannery wastewater in typical vadose zone in North China Plain[J]. Geology in China, 51(4): 1280−1289(in Chinese with English abstract).
[18]	Pantazopoulou E, Zouboulis A. 2018. Chemical toxicity and ecotoxicity evaluation of tannery sludge stabilized with ladle furnace slag[J]. Journal of Environmental Management, 216: 257−262. doi: 10.1016/j.jenvman.2017.03.077
[19]	Polti M A, Aparicio J D, Benimeli C S, Amoroso M J. 2014. Simultaneous bioremediation of Cr(Ⅵ) and lindane in soil by actinobacteria[J]. International Biodeterioration & Biodegradation, 88: 48−55.
[20]	Sabey B R, Frederick L R, Bartholomew W V. 1959. The formation of nitrate from ammonium mitrogen in soils: III Influence of temperature and initial population of nitrifying organisms on the maximum rate and delay period[J]. Soil Science Society of America, 23: 462−465. doi: 10.2136/sssaj1959.03615995002300060029x
[21]	Schloss P D, Westcott S L, Ryabin T, Hall J R, Hartmann M, Hollister E B, Lesniewski R A, Oakley B B, Parks D H, Robinson C J, Sahl J W, Stres B, Thallinger G G, Van H D J, Weber C F. 2009. Introducing mothur: Open–source, platform–independent, community–supported software for describing and comparing microbial communities[J]. Applied & Environmental Microbiology, 75(23): 7537−7541.
[22]	Solá M, Lovaisa N, Costa J, Benimeli C, Polti M, Alvarez, A. 2019. Multi–resistant plant growth–promoting actinobacteria and plant root exudates influence Cr(Ⅵ) and lindane dissipation[J]. Chemosphere, 222: 679−687. doi: 10.1016/j.chemosphere.2019.01.197
[23]	Wang B Z, Zhao J, Guo Z Y, Ma J, Xu H, Jia Z J. 2015. Differential contributions of ammonia oxidizers and nitrite oxidizers to nitrification in four paddy soils[J]. The ISME Journal, 9: 1062−1075. doi: 10.1038/ismej.2014.194
[24]	Wang Feng. 2006. Study on Function and Character of Nitrifying Bacteria in Sewage Treatment Processes[D]. Shanghai: Tongji University, 1–169 (in Chinese with English abstract).
[25]	Wang Ya'nan. 2007. Effects of Different Nitrogen Application Rates on Ammonia–oxidizing Bacteria Community Structure in Paddy Soil[D]. Beijing: China Agricultural University, 1–59 (in Chinese with English abstract).
[26]	Xu Ke, Chen Honghan. 2011. Progesson three forms of nitrogen contaminant transport and transform in groundwater[J]. China Population, Resources and Environment, 21(12): 421–424 (in Chinese with English abstract).
[27]	Yu Hao, An Yijun, Jin Decai, Jin Tuo, Wang Xingrun. 2021. Effects of chromium pollution on soil bacterial community structure and assembly processes[J]. Environmental Science, 42(3): 1197−1204 (in Chinese with English abstract).
[28]	Yu Ying. 2020. Hydroxylamine Promotes Nitrogen Removal and Microbial Mechanism during In–situ Aerobic Remediation of Landfill [D]. Shanghai: Shanghai University, 1–130. (in Chinese with English abstract).
[29]	Yuan Qiaoxia, Wu Yajuan, Ai Ping, Zhen Yucun, Zhu Duanwei. 2007. Effects of moisture, temperature and nitrogen supply rate on NO₃^––N accumulation in greenhouse soil[J]. Transactions of the Chinese Society of Agricultural Engineering, 23(10): 192−198 (in Chinese with English abstract).
[30]	Zeng J, Gou M, Tang Y Q, Li G Y, Sun Z Y, Kida K. 2016. Effective bioleaching of chromium in tannery sludgewith an enriched sulfur–oxidizing bacterial community[J]. Bioresource Technology, 218: 859–866.
[31]	Zeng Wei, Zhang Liming, Wang Anqi, Zhang Jie, Peng Yongzhen, Duan Junling. 2015. Community structures and population dynamics of nitrifying bacteria in activated sludges of wastewater treatment plants[J]. China Environmental Science, 35(11): 3257−3265 (in Chinese with English abstract).
[32]	Zhang Dazheng, Li Haiming, Zhan Xiaoyan, Xia Yuezhen. 2014. Characteristics of groundwater salt pollution in a typical leather–contaminated site[J]. Hydrogeology & Engineering Geology, 41(2): 18−23 (in Chinese with English abstract).
[33]	Zhang Jian. 2018. Effects of Continuous Nitrogen Application on Community Structure of Ammonia–oxidizing Flora and Bacteria on Potato–planting Soil[D]. Lanzhou: Gansu Agricultural University, 1–44 (in Chinese with English abstract).
[34]	畅浩. 2014. 制革污泥热解过程及其产物特性的研究[D]. 西安: 陕西科技大学, 1–52.
[35]	黄雪芬, 蒙敏, 谢刚, 罗宇晨, 李磊, 王维生. 2017. 制革污泥中Cr形态分布及Cr(Ⅵ)还原性研究[J]. 广西大学学报(自然科学版), 42(5): 1930−1936.
[36]	赖巧晖, 张浩, 刘治鹏. 2019. 不同植物配置下人工湿地微生物群落特征及其影响因素[J]. 水土保持研究, 26(5): 89−94,99.
[37]	李娟, Uwaremwe Constantine, 冷艳, 张晓华, 李师翁, 陈熙明. 2017. 节杆菌属细菌处理有机物和重金属污染物的研究进展[J]. 环境科学与技术, 40(10): 89−97.
[38]	李猛. 2017. 有机肥配施氮肥条件下设施菜地硝化菌群多样性研究[D]. 沈阳: 沈阳农业大学, 1–56.
[39]	吕晓立, 刘景涛, 韩占涛, 朱亮, 杨明楠, 李海军. 2021. 快速城镇化进程中珠江三角洲高铵地下水赋存环境及驱动因素[J]. 中国地质, 48(6): 1770−1780.
[40]	马宏瑞, 王晓蓉, 牛晓君, 李桂菊. 2004. 制革污泥污染土壤矿化过程中Cr(Ⅲ)和氮的释放[J]. 农业环境科学学报, 23(1): 136−139.
[41]	庞雅婕, 李春辉, 韩占涛, 张兆吉, 孔祥科. 2024. 华北平原制革废水Cr(III)和氨氮在典型包气带中迁移模拟与污染评价[J]. 中国地质, 51(4): 1280−1289.
[42]	王峰. 2006. 城市污水处理过程硝化菌群功能与群落特征研究[D]. 上海: 同济大学, 1–169.
[43]	王亚男. 2007. 不同施氮量对稻田土壤中氨氧化细菌群落结构的影响[D]. 北京: 中国农业大学, 1–59.
[44]	许可, 陈鸿汉. 2011. 地下水中三氮污染物迁移转化规律研究进展[J]. 中国人口·资源与环境, 21(12): 421−424.
[45]	于皓, 安益君, 金德才, 靳拓, 王兴润. 2021. 铬污染对土壤细菌群落结构及其构建机制的影响[J]. 环境科学, 42(3): 1197−1204.
[46]	喻颖. 2020. 填埋场原位好氧修复过程中羟胺促进脱氮效果及微生物作用机理研究[D]. 上海: 上海大学, 1–130.
[47]	袁巧霞, 武雅娟, 艾平, 甄玉存, 朱端卫. 2007. 温室土壤硝态氮积累的温度、水分、施氮量耦合效应[J]. 农业工程学报, 23(10): 192−198. doi: 10.3321/j.issn:1002-6819.2007.10.034
[48]	曾薇, 张丽敏, 王安其, 张洁, 彭永臻, 段俊岭. 2015. 污水处理系统中硝化菌的菌群结构和动态变化[J]. 中国环境科学, 35(11): 3257−3265. doi: 10.3969/j.issn.1000-6923.2015.11.008
[49]	张达政, 李海明, 詹晓燕, 夏跃珍. 2014. 典型制革污染场地地下水盐污染特征[J]. 水文地质工程地质, 41(2): 18−23.
[50]	张健. 2018. 连续施氮对土壤氨氧化菌及细菌群落结构的影响[D]. 兰州: 甘肃农业大学, 1–44.

施引文献

资源附件(0)

访问统计

图(5)

表(1)

计量

文章访问数: 195
PDF下载数: 62
施引文献: 0

中国地质调查局中国地质科学院	主办
科学出版社	出版

制革污泥渗滤液中特征污染物对土壤氨氮转化及微生物群落结构的影响

作者简介: 孔祥科，男，1987年生，副研究员，从事土壤、地下水污染机理与修复研究；E-mail：kongxiangke@mail.cgs.gov.cn

通讯作者: 王妍妍，女，1987年生，副研究员，主要从事地下水污染机理研究；E-mail：wangyanyan@mail.cgs.gov.cn。

Effects of characteristic pollutants in leather sludge leachate on soil ammonia nitrogen transformation and microbial community structures

Author Bio: KONG Xiangke, male, born in 1987, associate researcher, engaged in the mechanism and remediation of soil and groundwater pollution; E-mail:kongxiangke@mail.cgs.gov.cn .

Corresponding author: WANG Yanyan, female, born in 1987, associate researcher, engaged in the mechanism of groundwater pollution; E-mail: wangyanyan@mail.cgs.gov.cn.

计量

出版历程

目录