Optimization of Extraction Method for Easily Extractable Glomalin-Related Soil Protein from Calcareous Soil
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摘要:
在水-二氧化碳-碳酸盐岩-生物的相互作用下，全球形成8.24×108t C/a的岩溶碳汇，其中部分岩溶碳汇以土壤有机质的形式固存，进而在国家双碳目标中发挥重要作用。全球分布广泛的丛枝菌根真菌分泌的球囊霉素相关土壤蛋白(Glomalin-related soil protein，GRSP)性质稳定、不易分解，是土壤有机质的重要组成部分。提取合格的GRSP是深入研究岩溶土壤有机碳汇的基础，然而以往研究因GRSP提取量不高、提取不充分、产物不专一等问题，以至其在有机质中发挥的作用机制无法深入开展研究，因此，提高GRSP提取量对于探究岩溶土壤有机质的形成和稳定机制具有重要意义。本文选取岩溶区黑色、棕色、黄色和红色四种石灰土，通过温度和时间正交实验，筛选出适用于岩溶土壤颗粒态有机质(POM)和矿物结合态有机质(MAOM)易提取球囊霉素相关土壤蛋白(EE-GRSP)的最佳提取条件。实验结果表明，POM和MAOM在123℃和80min条件下提取时，EE-GRSP提取量最高。应用于四类石灰土后，EE-GRSP增量范围为4.6%～34.2%。相较于全土，MAOM具有更高的稳定性与更强的有机碳保护能力。因此，随着温度和提取时间的提高，MAOM中的EE-GRSP得到更完全的释放，提取量显著提高。由此可见，EE-GRSP提取条件优化对深入研究岩溶土壤碳汇潜力及其稳定机制具有重要意义。
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关键词:
- 易提取球囊霉素相关土壤蛋白 /
- 提取条件优化 /
- 颗粒态有机质 /
- 矿物结合态有机质
Abstract:Glomalin-related soil protein (GRSP) secreted by arbuscular mycorrhizal fungi is widely distributed worldwide, has stable properties and is not easily decomposed, which is an important component of soil organic matter. Extracting high quality GRSP is important for in-depth research on organic carbon sinks in calcareous soil from karst areas. However, in previous studies, the mechanism of GRSP in organic matter could not be further studied due to low extraction yield, insufficient extraction and non-specific products. Therefore, the high extraction amount of GRSP is of great significance to explore the formation and stabilization mechanism of organic matter in calcareous soil. This experiment selected four types of calcareous soil from karst areas: black, brown, yellow and red calcareous soil. By using orthogonal experiments of temperature and time, the optimal extraction conditions for easily extractable glomalin-related soil protein (EE-GRSP) related to particulate organic matter (POM) and mineral-associated organic matter (MAOM) in calcareous soil were selected. The experimental results showed that the highest extraction amount of EE-GRSP was achieved when POM and MAOM were extracted at 123℃ and 80min. After application into the four types of calcareous soil, the EE-GRSP contents increased from 4.6% to 34.2%. The BRIEF REPORT is available for this paper at
http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.yk202402060015 . -
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表 1 不同提取时间和提取温度下棕色石灰土、红色石灰土的EE-GRSP提取量
Table 1. EE-GRSP content of brown calcareous soil and red calcareous soil at different extracting time and temperatures
提取条件 EE-GRSP提取量(mg/g) 棕色石灰土-颗粒态有机质 红色石灰土-颗粒态有机质 棕色石灰土-全土 红色石灰土-全土 121℃，40min 0.456±0.070d 0.288±0.02de 0.7±0.028d 0.276±0.012cd 121℃，60min 0.688±0.045c 0.45±0.027b 0.659±0.039d 0.467±0.083a 121℃，80min 0.733±0.128bc 0.38±0.034c 0.89±0.082c 0.374±0.065abc 123℃，40min 0.641±0.083cd 0.347±0.023cd 0.91±0.074bc 0.397±0.062ab 123℃，60min 0.610±0.025cd 0.294±0.025de 1.05±0.041ab 0.321±0.066bcd 123℃，80min 0.963±0.054a 0.594±0.085a 1.126±0.114a 0.388±0.106ab 125℃，40min 0.581±0.052cd 0.269±0.019e 0.723±0.027d 0.256±0.014d 125℃，60min 0.892±0.021ab 0.387±0.027c 1.158±0.164a 0.439±0.01a 125℃，80min 0.729±0.237bc 0.306±0.016de 0.653±0.078d 0.302±0.005bcd 注：同列不同小写字母表示差异显著(p＜0.05)，含相同字母表示差异不显著。
Note: Different lowercase letters in the same column indicate significant difference (p＜0.05), and the same letters indicate no significant difference.
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