接菌对煤矸石重构土层结构的水盐分布及苜蓿生长的影响

刘庚辰, 柯增鸣, 毕银丽. 接菌对煤矸石重构土层结构的水盐分布及苜蓿生长的影响[J]. 水文地质工程地质, 2025, 52(4): 50-61. doi: 10.16030/j.cnki.issn.1000-3665.202412002
引用本文: 刘庚辰, 柯增鸣, 毕银丽. 接菌对煤矸石重构土层结构的水盐分布及苜蓿生长的影响[J]. 水文地质工程地质, 2025, 52(4): 50-61. doi: 10.16030/j.cnki.issn.1000-3665.202412002
LIU Gengchen, KE Zengming, BI Yinli. Effect of inoculation on water and salt distribution and alfalfa growth of coal gangue reconstructed layer[J]. Hydrogeology & Engineering Geology, 2025, 52(4): 50-61. doi: 10.16030/j.cnki.issn.1000-3665.202412002
Citation: LIU Gengchen, KE Zengming, BI Yinli. Effect of inoculation on water and salt distribution and alfalfa growth of coal gangue reconstructed layer[J]. Hydrogeology & Engineering Geology, 2025, 52(4): 50-61. doi: 10.16030/j.cnki.issn.1000-3665.202412002

接菌对煤矸石重构土层结构的水盐分布及苜蓿生长的影响

  • 基金项目: 国家自然科学基金重大项目(52394195);国家自然科学基金青年科学基金项目(52404187);国家重点研发计划项目(2022YFF1303304);陕西省自然科学基础研究计划项目(2024JC-YBQN-0588)
详细信息
    作者简介: 刘庚辰(2000—),男,硕士,主要从事矿区微生物复垦研究工作。E-mail:liugengc@163.com
    通讯作者: 毕银丽(1971—),女,博士,教授,主要从事矿区微生物复垦研究工作。E-mail:ylbi88@126.com
  • 中图分类号: TD167

Effect of inoculation on water and salt distribution and alfalfa growth of coal gangue reconstructed layer

More Information
  • 西部露天煤矿区排土场生态修复面临水资源严重匮乏的制约,三层(生态层-涵水层-隔水层)海绵结构能够起到涵蓄水分促进植物生长的作用,同时也存在持续浇灌咸水导致土壤盐碱化的风险。煤矸石作为煤炭开采产生的主要伴生固废,可资源化利用作为充填材料来改变复合材料的结构与性能,但将煤矸石作为三层海绵结构材料并联合微生物复垦盐碱化土壤的潜力尚不清楚。研究旨在探究三层海绵结构中不同涵水层材料(沙土、煤矸石)与接种丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)对土壤水盐分布及苜蓿生长的影响,采用室内模拟土柱试验方法,试验设置4组处理,分别为沙土涵水层未接菌(S-CK)、煤矸石涵水层未接菌(C-CK)、沙土涵水层接种AMF(S-AM)与煤矸石涵水层接种AMF(C-AM)。研究结果表明:(1)相较于S-CK处理,C-CK处理使表层土盐度降低了31.5%,使苜蓿地上部生物量增加了49.6%、地下部生物量增加了38.3%;(2)相较于C-CK处理,C-AM处理使苜蓿地上部生物量增加了3.8倍、地下部生物量增加2.5倍,使表层土盐度提高了24.7%,AMF促进了20~40 cm土层粗根系的发育;(3)煤矸石涵水层与接种AMF的协同作用,使生态层土壤含水率增加了16.5%,表层土盐度降低了14.5%,地上部生物量增加了4.1倍,地下部生物量增加了4.7倍。综合研究表明,煤矸石涵水层与接种AMF相结合可以营造适生的土壤水盐环境,有效降低土壤盐碱化风险。研究结果可为西部地区露天矿排土场生态修复与重建提供科学依据与技术支撑。

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  • 图 1  研究区位置图

    Figure 1. 

    图 2  三层结构剖面图

    Figure 2. 

    图 3  试验期间每10 d平均温度变化与采样时间点

    Figure 3. 

    图 4  试验装置图

    Figure 4. 

    图 5  不同处理下土壤水分分布特征(2023年)

    Figure 5. 

    图 6  不同处理下土壤盐分分布特征

    Figure 6. 

    图 7  不同处理下表层土壤盐分积累量

    Figure 7. 

    图 8  不同处理下0~10 cm与30~40 cm土壤孔隙度差异

    Figure 8. 

    图 9  不同处理下苜蓿地上部与地下部生物量

    Figure 9. 

    图 10  相关性分析热图(字母q、s分别表示0~10 cm与30~40 cm土层)

    Figure 10. 

    表 1  煤矸石主要化学成分及质量百分比

    Table 1.  Main chemical composition and mass percentage of coal gangue

    化学成分 质量占比/% 化学成分 质量占比/%
    Na2O 1.0216 Fe2O3 6.5635
    MgO 1.6334 NiO 0.0067
    Al2O3 21.2891 CuO 0.0066
    SiO2 62.0793 ZnO 0.0123
    P2O5 0.1121 Ga2O3 0.0038
    SO3 0.7712 As2O3 0.0031
    Cl 0.0187 Rb2O 0.0194
    K2O 3.6095 SrO 0.0338
    CaO 1.6998 Y2O3 0.0032
    TiO2 0.9076 ZrO2 0.0206
    Cr2O3 0.0177 Nb2O5 0.002 0
    MnO 0.0947 BaO 0.0701
    下载: 导出CSV

    表 2  土柱填充基质物理性质

    Table 2.  Physical Properties of Filling Substrate of Soil Column

    土层
    结构
    土壤
    质地
    容重
    /(g·cm−3
    粒径范围及比例 孔隙度
    /%
    田间持
    水量/%
    生态层 黄土∶沙土
    (质量比)=
    1∶5
    1.47 <0.002 mm,2.08%
    0.002~0.050 mm,30.74% 23.2 15.6%
    0.050~2.000 mm,67.18%
    涵水层 煤矸石 1.27 0.5~3.0 cm 42.3
    沙土 1.42 0~2.000 mm 20.0
    下载: 导出CSV

    表 3  不同处理的苜蓿侵染率

    Table 3.  Infection rate of alfalfa under different treatments

    分组C-CKS-CKC-AMS-AM
    侵染率/%3.7±2.25.2±3.061.5±6.765.2±5.9
    下载: 导出CSV

    表 4  不同处理下苜蓿根系分布特征

    Table 4.  Distribution characteristics of alfalfa roots under different treatments

    土层深度/cm RLD/(cm·cm−3 RVD/(cm3·cm−3 RSD/(cm2·cm−3
    涵水层处理 CK AM CK AM CK AM
    0~20 S 0.08Ba 0.12Ab 0.06Ba 0.25Aa 0.19Ba 0.49Ab
    C 0.10Ba 0.31Aa 0.07Ba 0.36Aa 0.24Ba 0.85Aa
    20~40 S 0.12Aa 0.09Aa 0.02Ba 0.09Aa 0.16Ba 0.26Aa
    C 0.11Aa 0.15Aa 0.03Ba 0.08Aa 0.16Ba 0.33Aa
    0~10 0.09ab 0.23a 0.09a 0.36a 0.26a 0.76a
    10~20 0.08b 0.20a 0.04b 0.26a 0.17bc 0.58a
    20~30 0.14a 0.11a 0.04bc 0.09b 0.2ab 0.27a
    30~40 0.09ab 0.14a 0.02c 0.08b 0.11c 0.31a
    ANOVA P-value
    土层深度 0.032 0.617 0.006 0.023 0.018 0.156
    涵水层 (0~20) Ns * Ns Ns Ns *
    涵水层 (20~40) Ns Ns Ns Ns Ns Ns
      注:不同大写字母代表涵水层中CK与AM处理之间的差异显著,不同小写字母代表同一土层深度中沙土涵水层(S)与煤矸石涵水层(C)之间的差异显著;土层深度中不同小写字母代表同一列不同深度下的数据差异显著,(Duncan检验,P<0.05);Ns代表不同处理之间无显著性差异,*代表不同处理之间存在显著性差异(P<0.05)。
    下载: 导出CSV
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出版历程
收稿日期:  2024-12-01
修回日期:  2025-03-24
刊出日期:  2025-07-15

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