高效液相色谱-质谱法测定不同产地不同部位烟叶中茄尼醇
Determination of Solanesol in Different Parts of Tobacco Leaves from Different Breeds and Planting Areas by High Performance Liquid Chromatography-Mass Spectrometry
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摘要: 对不同产地、不同品种和不同部位烟叶中茄尼醇的含量进行测定比较，以考察烟叶产地、品种及部位与茄尼醇含量的关系。烟叶样品进行皂化及超声提取处理后，采用反相高效液相色谱法（RP—HPLC）、大气压化学电离质谱法（APCI—MS）进行定性、定量测定。方法测定茄尼醇的线性范围为41．2～6180ng（相关系数为0．9999），0．412g／L的茄尼醇标准溶液5次重复测定的保留时间的精密度（RSD）为0．08％，峰面积的精密度为0．20％；方法回收率为83．1％～103．9％，波长211nm处的检测限为3．72ng。样品测定结果显示，不同部位烟叶的茄尼醇生产能力明显不同，同一烟叶自上而下各部位中茄尼醇含量逐渐降低，烟梗中茄尼醇含量极低。在以烟叶为原料生产茄尼醇时，应去除烟梗；并考虑选择烟叶中茄尼醇含量高的产区作为原料供应基地。
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关键词:
- 茄尼醇 /
- 高效液相色谱-质谱法 /
- 烟叶
Abstract: In this article, the contents of solanesol in different parts of tobacco leaves, from different breeds and different planting areas were determined. The solanesol was extracted from tobacco leaves via saponification and ultrasonic assist extraction and determined by RP-HPLC and APCI-MS with linear range of 41.2～6180 ng(R2=0.9999). The precision of the retention time is 0.08%RSD (n=5) and the precision of the peak area determination is 0.20%RSD (n=5). The recovery of the method is 83.1%～103.9% with the detection limit of 3.72 ng at 211 nm for solanesol. The results indicate that the contents of solanesol in different parts of tobacco leaves are distinctly different. The content of solanesol in a single tobacco leave drops from the top part to the nether part. The content of solanesol in the peduncle is very low. In order to increase the production and cut the cost, the obacco leaves with higher solanesol should be selected for solanesol production. -
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