Abstract: The rapid social and economic development and the trend of human migration to large- and mid-size cities, especially provincial capitals, have significantly intensified land use conflicts. The coordination among production, living, and ecological spaces is significant for sustainable, regional social and economic development. This study created a multi-purpose suitability assessment model from the perspective of the production, living, and ecological functions, identified the production, living, and ecological suitability, as well as the intensity of potential land use conflicts, in Nanchang City, China while considering land space background and planning objectives for differentiated regional regulation. The results indicate that over 65% of areas in the city are suitable for production and living. Areas with ecological, productive, and living suitability differ in spatial distribution and structural composition and exhibit pronounced overlaps. This indicates potential land use conflicts. The conflict identification results reveal that the areas with severe, strong, moderate, and weak land use conflicts account for 0.53%, 18.81%, 5.77%, and 5.67%, respectively. Given the different spatial distributions, area proportions, and characteristics of areas with potential land use conflicts, differentiated regulations are required. Based on comprehensive considerations of the conflict identification results and the functional zoning stated in the Nanchang City Land and Space Master Plan (2021-2035), this study determined nine major zones for differentiated regulation. This study made some preliminary attempts in zoning regulation against land use conflicts while considering both land use suitability and the requirements for social and economic development. The results of this study will provide a scientific basis for identifying land use conflicts and optimizing land space layout in other similar cities.