Abstract: Copper resource in China is abundant,but the quality of this copper resources is unsatisfactory and the copper resources is still a shortage minerals resources in domestic.Realizing the efficient development and full use of low grade copper ore resources has vital significance for the stable development of copper industry economy in our country.Copper sulfur flotation separation was studied from a low grade cooper-bearing gold ore,in which the grade of cooper,gold and sulfur were 0.501%,0.4 g/t and 3.88% respectively.The Cooper minerals were mainly in the form of copper sulfide,which reached 96% of the total copper.Mosaic size of cooper minerals in it was quite fine,and occurred together dense with pyrite.Based on detailed experiments of flotation condition (including grinding fineness,collector dosage,CaO dosage and grinding fineness during copper sulfur flotation separation) and flow sheet test of technology (including scavenging times and cleaning times).Following results were obtained:the grinding fineness of roughing was 58.13% below 75 μm,the slurry pH value is about 6 with 100 g/t of copper sulfate as the activator,using butyl xanthate and butyl amine black reagent for combination collector with a dosage of 300 g/t(butyl xanthate:butyl amine black reagent =2 ∶ 1),the grinding fineness of copper and sulphur separation was 82.45 % below 45 μm with 2 kg/t of lime as the depressor and adopting a roughing,twice scavenging and three times of cleaning processing.The flotation process of" stage grinding-mixed flotation-copper sulfur separation" was finally adopted to realize the effective recovery of copper,gold and sulfur.Closed circuit test results showed that the grade of copper concentrate was about 19%,the content of gold was about 9.5 g/t and the recovery of copper and gold were Cu78% and Au50% respectively.This flotation process is simple and the production cost is low,which can be provided for technical reference and decision-making basis for the next step construction of copper plant.