Abstract: Due to the difficulty to separate jamesonite and galena by flotation,it is the often case to melt the bulk concentrate,which often causes high smelting cost.To separate the two minerals efficiently,flotation behavior of jamesonite and galena was investigated with sodium diethyldithiocarbamate (DDTC)and ammonium dibutyl dithio phosphate (ADDP) as collectors respectively under different pulp condition.Then conditional tests of real ore and whole process open-circuit test were carried out to further confirm the actual separation conditions of the two minerals.It was shown that ammonium dibutyl dithiophosphate has good collecting performance both on galena and jamesonite.But DDTC has better selectivity and collecting ability on galena than on jamesonite in high alkaline environment.Infrared spectroscopic analysis showed that chemical adsorption of DDTC took place on the surfaces of both galena and jamesonite.But the adsorption on galena is stronger and adsorption product is more stable.